Author(s): N. Sunitha, Y. Sujitha, B. Thangabalan, S. Manohar Babu

Email(s): sujithayarlagadda@gmail.com

DOI: Not Available

Address: N. Sunitha, Y. Sujitha*, B. Thangabalan, S. Manohar Babu
Department of Pharmaceutical Analysis, SIMS College of Pharmacy, Mangaldas Nagar, Guntur-522 002 (A.P.)
*Corresponding Author

Published In:   Volume - 6,      Issue - 1,     Year - 2014


ABSTRACT:
A simple, fast, precise, selective and accurate RP-HPLC method was developed and validated for the simultaneous determination of Ketorolac tromethamine and olopatadine HCl from bulk and formulations. Chromatographic separation was achieved isocratically on a Inertsil ODS C18 column (250×4.6 mm, 5 µ particle size) using a mobile phase 0.1 M Sodium di hydrogen orthophosphate: Acetonitrile in the ratio of 55:45. The flow rate was 1 ml/min and effluent was detected at 235nm. The retention time of Ketorolac and olopatadine were 2.507 min and 4.933 min. respectively. Linearity was observed in the concentration range of 4-24µg/ml and 12-72µg/ml for Ketorolac and olopatadine respectively with correlation coefficient of 0.999 for both the drugs. Percent recoveries obtained for ketorolac and olopatadine were 100.03% and 100.04%, respectively. The method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness. The method developed can be used for the routine analysis of Ketorolac and olopatadine from their combined dosage form.


Cite this article:
N. Sunitha, Y. Sujitha, B. Thangabalan, S. Manohar Babu. Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Ketorolac Tromethamine and Olopatadine Hydrochloride in Pure and Pharmaceutical Formulation. Res. J. Pharm. Dosage Form. and Tech. 6(1): Jan.-Mar. 2014; Page 37-43.

Cite(Electronic):
N. Sunitha, Y. Sujitha, B. Thangabalan, S. Manohar Babu. Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Ketorolac Tromethamine and Olopatadine Hydrochloride in Pure and Pharmaceutical Formulation. Res. J. Pharm. Dosage Form. and Tech. 6(1): Jan.-Mar. 2014; Page 37-43.   Available on: https://rjpdft.com/AbstractView.aspx?PID=2014-6-1-7


Recomonded Articles:

Author(s): Gopal Rao M., M. A. Amutha Gnana Arasi, S. Jayaprakash, M. Arun Kumar, P. Kavitha

DOI:         Access: Open Access Read More

Author(s): B. Thangabalan, Ch. Sandhya, N. Sunitha, S. Manohar Babu

DOI:         Access: Open Access Read More

Author(s): Shrikrishna B. Baokar, Sugandha V. Mulgund, Nisharani S. Ranpise

DOI:         Access: Open Access Read More

Author(s): Abd El-Aziz B. Abd El-Aleem, Shaban M. Khalile, Amr M. Badawy, Omneya K. El-Naggar

DOI:         Access: Open Access Read More

Author(s): T. Gopi Raju, M. Gayatri Ramya, A. Rajesh, S. Kathirvel, Sanjay Kumar Gupta

DOI:         Access: Open Access Read More

Author(s): Prajkta M. Ghagare, Ashwini R. Patil, Bhavna J. Deshmane, Manish S. Kondawar

DOI: 10.5958/0975-4377.2020.00004.X         Access: Open Access Read More

Author(s): Santanu Ghosh , BB Barik

DOI:         Access: Open Access Read More

Author(s): Pankaj Savani, Vineet Jain, Hasumati Raj, Sagar Patel

DOI: 10.5958/0975-4377.2016.00009.4         Access: Open Access Read More

Author(s): Abd El-Aziz B. Abd El-Aleem, Shaban M. Khalile, Amr M. Badawy, Omneya K. El-Naggar

DOI:         Access: Open Access Read More

Author(s): L. Satyanarayana, S.V. Naidu, M. Narasimha Rao, C. Ayyanna, Alok Kumar

DOI:         Access: Open Access Read More

Author(s): Mohamad Azhar A. Patwekar, V.R. Salunkhe

DOI:         Access: Open Access Read More

Author(s): Chetan Salunke, Kailaspati Chittam, Amol Pawar, Harshal Chaudhari

DOI: 10.5958/0975-4377.2020.00016.6         Access: Open Access Read More

Author(s): Swapna Singh, Prem Kumar Bichala, Abhishek Agrawal

DOI: 10.52711/0975-4377.2021.00037         Access: Open Access Read More

Author(s): S. Janet Beula, T. Ramamohan Reddy, R. Suthakaran, M. Viswaja

DOI: 10.52711/0975-4377.2023.00030         Access: Open Access Read More

Research Journal of Pharmaceutical Dosage Forms and Technology (RJPDFT) is an international, peer-reviewed journal, devoted to pharmaceutical sciences. ...... Read more >>>

RNI: Not Available                     
DOI: 10.5958/0975-4377 


Recent Articles




Tags