Author(s): Chetan Salunke, Kailaspati Chittam, Amol Pawar, Harshal Chaudhari

Email(s): amolraje957@gmail.com

DOI: 10.5958/0975-4377.2020.00016.6   

Address: Chetan Salunke*, Kailaspati Chittam, Amol Pawar, Harshal Chaudhari
Department of Pharmacognosy, Annasaheb Ramesh Ajmera College of Pharmacy Nagao Dhule, Dist: Dhule 424002, Maharashtra, India.
*Corresponding Author

Published In:   Volume - 12,      Issue - 2,     Year - 2020


ABSTRACT:
A new, simple, sensitive, selective, precise and robust high-performance thin-layer chromatographic (HPTLC) method for analysis of glycyrrhizin has been developed and validated for the determination of glycyrrhizin in herbal extracts. The analyte was extracted with ethanol and applied on TLC aluminium plates along with standard using Linomat 5 spray on sample applicator (CAMAG). Analysis of glycyrrhizin was performed on pre-coated TLC aluminium plates with silica gel as the stationary phase and prewashed with methanol. Linear ascending development was carried out in twin trough glass chamber saturated with mobile phase consisting of ethyl acetate-methanol-water-formic acid (15:2:1:1 v/v/v/v). Spectrodensitometric scanning was performed by TLC scanner III (CAMAG) in absorbance mode at the wavelength of 252nm. The system was found to give compact spots for glycyrrhizin (Rf value of 0.34 ± 0.04). The linear regression analysis data for the calibration plots showed good linear relationship with r2=0.9981 in the concentration range 2-15 µl with respect to peak area. According to the International Conference on Harmonization (ICH) guidelines the method was validated for precision, recovery, stability in solution and robustness. The glycyrrhizin content quantified from herbal extracts and from the formulation was found well within limits. Statistical analysis of the data showed that the method is reproducible and selective for the estimation of glycyrrhizin.


Cite this article:
Chetan Salunke, Kailaspati Chittam, Amol Pawar, Harshal Chaudhari. Development of an Analytical Method to Determine Extracts of Different Species of Glycyrrhizin by HPTLC. Res. J. Pharma. Dosage Forms and Tech.2020; 12(2): 89-92. doi: 10.5958/0975-4377.2020.00016.6


Recomonded Articles:

Author(s): Yamunappa, Ravi Kumar, Pooja Shetty, Prathibha Suvarna, Narayana Swamy VB

DOI: 10.5958/0975-4377.2016.00012.4         Access: Open Access Read More

Author(s): Gopal Rao M., M. A. Amutha Gnana Arasi, S. Jayaprakash, M. Arun Kumar, P. Kavitha

DOI:         Access: Open Access Read More

Author(s): Ashok A Hajare, Mahesh N Mali, Arun S Dange, Sushil M Sarvagod, Shweta V Patwardhan ,Sachin T Kurane

DOI:         Access: Open Access Read More

Author(s): Shubham Tripathi, Somnath Patel, Ritesh Patel, Shraddha,Pushpendra, Sachin,Neetish, Mahendra, Kuldeep, Prashant, Rajesh Patel, Girish, Nitin, Tomanlal, Amrita Thakur, Mukta Agrawal, Ajazuddin, D. K. Tripathi, Amit Alexander

DOI: 10.5958/0975-4377.2018.00019.8         Access: Open Access Read More

Author(s): Sandeep Gupta, Dheeraj Ahirwar, Neeraj K Sharma, Deenanath Jhade

DOI:         Access: Open Access Read More

Author(s): M.S. Gambhire, S. Lilke , V.M. Gambhire

DOI:         Access: Open Access Read More

Author(s): Ayushmaan Roy. Anjali Wahane, Siddharth Karankal, Prachi Sharma, Davesh Khutel, Onkarnath Singh, Vinay Shardul, Pitamber, Nausheen Sabha, Jyoti Dewangan, Ajay Dewangan, Akash Jangde, Chaya Rani, Tekeshwar Sahu, D. K. Tripathi, Mukta Agrawal, Ajazuddin, Gyanesh Sahu, Amit Alexander

DOI: 10.5958/0975-4377.2018.00012.5         Access: Open Access Read More

Author(s): Banhishikha Kar, Ayan Kumar Kar

DOI: 10.5958/0975-4377.2018.00008.3         Access: Open Access Read More

Author(s): Vidya N. Dange, Shubhangi J. Shid, Sagar B. Patil, Ganesh B. Vambhurkar, Mangesh A. Bhutkar

DOI: 10.5958/0975-4377.2019.00010.7         Access: Closed Access Read More

Author(s): Varsha R. Sandhan, S.B. Gondkar, R. B. Saudagar

DOI:         Access: Open Access Read More

Author(s): Suraj R. Wasankar, Kshitij V. Makeshwar, Abhishek D. Deshmukh, Rahul M. Burghate

DOI:         Access: Open Access Read More

Author(s): P. B. Suruse, M. K. Kale, N. J. Duragkar, A. Gundawar

DOI:         Access: Open Access Read More

Author(s): Akelesh T., R. Ramya, Venkatesh. P., Divya Jacob , R. Venkatnarayanan

DOI:         Access: Open Access Read More

Author(s): Nikita S. Malekar, S.B. Gondkar, R.B. Saudagar

DOI: 10.5958/0975-4377.2015.00040.3         Access: Open Access Read More

Author(s): Baladaniya Manoj, Vadgama Neha, Patel Priya

DOI:         Access: Open Access Read More

Author(s): Deelip V Derle, Sagar BSH , Devendra R Yeole

DOI:         Access: Open Access Read More

Research Journal of Pharmaceutical Dosage Forms and Technology (RJPDFT) is an international, peer-reviewed journal, devoted to pharmaceutical sciences. ...... Read more >>>

RNI: Not Available                     
DOI: 10.5958/0975-4377 


Recent Articles




Tags