L. Satyanarayana, S.V. Naidu, M. Narasimha Rao, C. Ayyanna, Alok Kumar
L. Satyanarayana1*, Prof. S.V. Naidu2, Prof. M. Narasimha Rao2, Prof. C. Ayyanna2 and Alok Kumar1.
1Department of Pharmaceutical Chemistry, Omega College of Pharmacy, Edulabad, Ghatkesar, Ranga Reddy Dist., Hyderabad-500 034.
2Centre for Biotechnology, Department of Chemical Engineering, College of Engineering (A), Andhra University, Vishakapatnam - 530003
Volume - 3,
Issue - 5,
Year - 2011
A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Maraviroc in tablet dosage form. A RP Inertsil ODS-3V C-18, 250x4.6 mm, 5µm partical size, with mobile phase consisting of 0.02M Dipotasium hydrogen orthophosphate in water pH 2.5 with orthophosphoric acid and Acetonitrile in the ratio of 60:40 v/v was used. The flow rate was 0.8 ml/min and the effluents were monitored at 210 nm. The retention time was 4.330 min. The detector response was linear in the concentration of 80-240µg/ml. The respective linear regression equation being Y= 31018.059X + 44222.4. The limit of detection and limit of quantification was 0.2µg and 0.6µg/ml respectively. The percentage assay of Maraviroc was 99.42%. The method was validated by determining its accuracy, precision and system suitability.
The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Maraviroc in bulk drug and in its pharmaceutical dosage form.
Cite this article:
L. Satyanarayana, S.V. Naidu, M. Narasimha Rao, C. Ayyanna, Alok Kumar. The Estimation of Maraviroc in Tablet dosage form by RP-HPLC. Research J. Pharma. Dosage Forms and Tech. 2011; 3(5): 230-232 .