Development of Stability Indicating RP-HPLC Method for Estimation of Clemastine Fumarate and its Pharmaceutical Dosage Form

Ingole L. R.

doi:10.5958/0975-4377.2019.00014.4

Research Journal of Pharmaceutical Dosage Forms and Technology

ISSN

0975-4377 (Online)
0975-234X (Print)

Submit Article

Development of Stability Indicating RP-HPLC Method for Estimation of Clemastine Fumarate and its Pharmaceutical Dosage Form

Author(s): Ingole L. R.

Email(s): leenaingole2016@gmail.com

DOI: 10.5958/0975-4377.2019.00014.4

Address: Ingole L. R.
Ravi Institute of Diploma in Pharmacy, Koradi, Nagpur
*Corresponding Author

Published In: Volume - 11, Issue - 2, Year - 2019

View HTML

View PDF

ABSTRACT:
A simple, fast and precise stability indicating RP-HPLC method was developed for quantification of Clemastine Fumarate in pure and pharmaceutical dosage form. The quantification was carried out using LiChrospher® 100 RP-C8 endcapped column (5µm,150 x 4.6mm) and mobile phase comprised of methanol and water (0.05% triethyamine) in proportion of ratio 90:10 and degassed under ultrasonication. The flow rate was 0.8ml/min and effluent was monitored at 220nm. The retention time of Clemastine Fumarate was found to be 7.49±0.039 min and peak was separated. The method was validated in terms of linearity, precision, accuracy, specificity, robustness, limit of detection and limit of quantitation in accordance with ICH guideline. Linearity of Clemastine Fumarate was in the range 15-50µg/mL. The percentage recovery of Clemastine Fumarate were 98.46 to 99.99% from tablet formulation. The stability –indicating capability was established by forced degradation experiments. The proposed method suitable for determination of Clemastine Fumarate in pharmaceutical dosage form.

Keywords:

Cite this article:
Ingole L. R. Development of Stability Indicating RP-HPLC Method for Estimation of Clemastine Fumarate and its Pharmaceutical Dosage Form. Res. J. Pharma. Dosage Forms and Tech.2019; 11(2): 87-94. doi: 10.5958/0975-4377.2019.00014.4

Cite(Electronic):
Ingole L. R. Development of Stability Indicating RP-HPLC Method for Estimation of Clemastine Fumarate and its Pharmaceutical Dosage Form. Res. J. Pharma. Dosage Forms and Tech.2019; 11(2): 87-94. doi: 10.5958/0975-4377.2019.00014.4 Available on: https://rjpdft.com/AbstractView.aspx?PID=2019-11-2-5

Recomonded Articles:

Pharmaceutical Suspensions: Pharmacist has the Very Best Possible Formulation for the Job at Hand.

Author(s): Praneta Desale

DOI: Access: Open Access Read More

Factorial Designing for Pharmaceutical Product and Process Development

Author(s): Vinod K.R., Sandhya S

DOI: Access: Open Access Read More

Formulation Development and Evaluation of Capsule Containing Fluclaxacillin Sodium and Amoxicillin Trihydrate with Enteric Coated Granules of Lactic Acid Bacillus

Author(s): Gopal Rao M., M. A. Amutha Gnana Arasi, S. Jayaprakash, M. Arun Kumar, P. Kavitha

DOI: Access: Open Access Read More

Pharmaceutical Packaging Technology: A Brief Outline

Author(s): Pratik Swarup Das, Puja Saha1, Krishan, Rumpa Das

DOI: :10.5958/0975-4377.2018.00005.8 Access: Open Access Read More

Formulation, Evaluation of Clotrimazole Vaginal Suppository

Author(s): V. S. Parmar, S.C. Shivhare

DOI: Access: Open Access Read More

Cleaning Validation of Paracetamol Tablets as a Dosage Formulation

Author(s): Hapse S.A., Bhagat B.V., Wagh V.S. , Kadaskar P.T.

DOI: Access: Open Access Read More

Transdermal Drug Delivery System of Antidiabetic Drugs: A Review

Author(s): Swapnil T. Deshpande, P. S. Vishwe, Rohit D. Shah, Swati S. Korabu, Bhakti R. Chorghe, DG Baheti

DOI: Access: Open Access Read More

Formulation and Evaluation of Stable Montelukast Sodium Sublingual Tablet by using Lyophilization Technique

Author(s): Dipti G. Phadtare, Amol R. Pawar, R.B. Saudagar, Govind K.Patil

DOI: 10.5958/0975-4377.2017.00002.7 Access: Open Access Read More

Isolation and evaluation of Basella alba linn. Leaf mucilage as release retardant in tablet formulation

Author(s): Yamunappa, Ravi Kumar, Pooja Shetty, Prathibha Suvarna, Narayana Swamy VB

DOI: 10.5958/0975-4377.2016.00012.4 Access: Open Access Read More

A Validated Method for the Estimation of EDTA in Drug Substances and their Intermediates by using Reversed Phase High Performance Liquid Chromatography

Author(s): Dharmendra Kumar Kushwah, Prakash Yashwant Kohle, Bimal Kumar Srivastava, Manoj Parmar ,Dhaval Mehta

DOI: Access: Open Access Read More

Formulation and Evaluation of Fast Dissolving Tablets of Trimetazidine Dihydrochloride Using Natural and Synthetic Superdisintegrants

Author(s): Simila Madathil, RaviKumar, Anju Govind, Mercy Mathew, Narayana Swamy VB

DOI: 10.5958/0975-4377.2016.00013.6 Access: Open Access Read More

Sensitive Visible Spectrophotometric methods Development for Estimation of Sapropterin Dihydrochloride in tablet dosage form

Author(s): U. Viplava Prasad, M. Syam Bab, B. Kalyana Ramu

DOI: 10.5958/0975-4377.2017.00007.6 Access: Open Access Read More

Analytical method development and validation for Estimation of Calcium Dobesilate and Docusate Sodium in Pharmaceutical dosage form with QbD approach

Author(s): Jaydeep S Chauhan, Ritika Gajre

DOI: 10.52711/0975-4377.2021.00038 Access: Open Access Read More

Research Journal of Pharmaceutical Dosage Forms and Technology (RJPDFT) is an international, peer-reviewed journal, devoted to pharmaceutical sciences. ...... Read more >>>

RNI: Not Available
DOI: 10.5958/0975-4377