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Research Journal of Pharmaceutical Dosage Forms and Technology
ISSN: 0975-4377(Online), 0975-234X(Print)
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Production of Industrially Significant Enzymes from Bio-Wastes Using Aspergillus niger by Solid State Fermentation
Fanish K. Pandey
Manoj K. Sharma
Tara Chand1, Fanish K. Pandey2, Shruti Dhingra3, Manoj K. Sharma4*
1CMJ University, Meghalaya,
2Noida International University, Greater Noida
3Delhi University, New Delhi,
4Head of Microbiology Lab., Dabur India Ltd. Ghaziabad
In this study the production of cellulases from Aspergillus niger by solid state fermentation was performed using bio wastes viz; sugarcane bagasse and wheat bran. One factor at a time approach was used to optimize the variables such as pH, substrate ratio and temperature. A maximum of 988.2 IU/gm activity of enzyme is observed at substrates ratio of 2:1 (sugarcane bagasse: wheat bran) at 30°C and pH 5 after 5 days incubation. The high cost of production of Cellulotic enzymes has hindered the industrial application of cellulose bioconversion. Hence, this study showed an effective utilization of sugarcane bagasse for the production of cellulotic enzymes which could be effectively used in degradation of cellulose into smaller glucose units in a cost effective manner.
Sugarcane bagasse, Wheat bran, Aspergillus niger, cellulase, cellulose.
Tara Chand, Fanish K. Pandey, Shruti Dhingra, Manoj K. Sharma. Production of Industrially Significant Enzymes from Bio-Wastes Using Aspergillus niger by Solid State Fermentation. Res. J. Pharm. Dosage Form. and Tech. 6(1): Jan.-Mar. 2014; Page 33-36.
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