Author(s): Mahawar Sheetal

Email(s): Email ID Not Available

DOI: Not Available

Address: Mahawar Sheetal*
Department of Pharmacy, Shri Jagdish Prasad Jhabarmal Tibrewala University, Vidyanagari, Jhunjhunu, Rajasthan, India
*Corresponding Author

Published In:   Volume - 5,      Issue - 3,     Year - 2013


ABSTRACT:
The present study investigates the formulation of pH-sensitive liposomes of docetaxel prepared by TFH technique using membrane composition consisting of one or combination of lipids such as DOPE, HSPC, CHOL, CHEMS and Cholesterol. Optimized process parameters evaluated are composition of solvent system (CHCl3:MeOH) (2:1), vacuum applied (600mmHg), solvent evaporation time (60 min), speed of rotation (100 rpm), hydration time (60 min), hydration volume (2 ml), no. of sonication cycles (30 sec) [(3 cycles (80% amp, 0.6 cycle)]. Optimized formulation parameters evaluated are Drug: Lipid ratio (1:20), hydration media (HEPES buffer pH 8.2), LIPID: CHOL ratio (8:2), LIPID: CHOL: CHEMS (6:2:2), HSPC: DOPE (1:3). The particle size of optimized formulation was found to be 111.0 nm ± 2.713nm with PDI of 0.227. Zeta potential of optimized formulation was -22.8 mV, attributed to the anionic nature of lipid. Percentage entrapment efficiency of optimized liposomal formulation was 64.79±0.230. Based on the DSC study of lyophilized docetaxel loaded liposome, melting endotherm for docetaxel was not observed, which indicates that docetaxel in the liposome was in an amorphous or disordered-crystalline phase of molecular dispersion or a solid state in the lipid layer of liposome. The surface morphology of lyophilized pH-sensitive liposomes exhibits a spherical shape of liposome. For preparing dry solid liposomal formulation, Trehalose was found more effective cryoprotectant showing highest PDR (92.76±0.571) and better size (173.4±3.721). From the In vitro diffusion study, it was concluded that the pH-sensitive liposomes shows higher pH-sensitivity since it release more amount of drug at pH 5.0. The cytotoxicity of docetaxel in conventional and pH-sensitive liposome on lung cancer cells, A549, was compared with that plain docetaxel by MTT assay. The higher cytotoxicity of pH-sensitive liposome than that of conventional liposome and plain drug was found mainly due to increase in the intracellular release of the drug from the pH-sensitive liposome at endosomal pH and avoiding the lysosomal degradation of the drug.


Cite this article:
Mahawar Sheetal. Development and Characterization of Docetaxel Encapsulated pH-Sensitive Liposomes for Cancer Therapy. Research J. Pharma. Dosage Forms and Tech. 2013; 5(3): 151-160 .


Recomonded Articles:

Author(s): Dipti G. Phadtare, Amol R. Pawar, R.B. Saudagar, Govind K.Patil

DOI: 10.5958/0975-4377.2017.00002.7         Access: Open Access Read More

Author(s): Dilip M. Kumbhar, Kailas K. Mali, Remeth J. Dias, Vijay D. Havaldar, Vishwajeet S. Ghorpade, Nitin H. Salunkhe.

DOI: 10.5958/0975-4377.2016.00036.7         Access: Open Access Read More

Author(s): Santosh J Pawar, Pruthviraj S Pawar, Sachin B Jadhav, Alkunte AS , Patil SM

DOI:         Access: Open Access Read More

Author(s): Sandhya Raj S., Sundramoorthy. K., Vetrichelvan. T.

DOI:         Access: Open Access Read More

Author(s): Sethi RK, Sahoo SK, Das PK, Barik BB

DOI:         Access: Open Access Read More

Author(s): Rathor Shruti, Ram Alpana

DOI:         Access: Open Access Read More

Author(s): Venkatesh Gavini, M. Srinivasa Murthy, P. Kiran Kumar ,D. L. Radhika

DOI:         Access: Open Access Read More

Author(s): N. Sandhya Rani, M. Teja Krishna, V. Saikishore.

DOI:         Access: Open Access Read More

Author(s): SM Sarode, MK Kale, G Vidyasagar

DOI:         Access: Open Access Read More

Author(s): T.V.Rao, S.Vidhyadhara, K.R.S. Sambasivarao

DOI:         Access: Open Access Read More

Author(s): S. M. Sarode, M. K. Kale, G. S. Talele, G. Vidyasagar

DOI:         Access: Open Access Read More

Author(s): M. Surendra, T. Venkateswara Rao, K. Lokeswara Reddy, A. Ramesh Babu

DOI:         Access: Open Access Read More

Author(s): K.M. Lokamatha Swamy, D. Manjula, S.M. Shanta Kumar, N. Rama Rao, Somshekar Shyale, Suma R.

DOI:         Access: Open Access Read More

Author(s): N. Sandhya Rani, M. Teja Krishna, V. Saikishore

DOI:         Access: Open Access Read More

Author(s): Priya D. Khode, Tina B. Katre

DOI: 10.5958/0975-4377.2019.00034.X         Access: Closed Access Read More

Research Journal of Pharmaceutical Dosage Forms and Technology (RJPDFT) is an international, peer-reviewed journal, devoted to pharmaceutical sciences. ...... Read more >>>

RNI: Not Available                     
DOI: 10.5958/0975-4377 


Recent Articles




Tags