Author(s): M. Venkata Ramana, V. Sowmya, K. Sangeetha, K. Nagapreethi, M. Mounika, S. Divya, A. Kanakalaxmi

Email(s): sowmyalucky72@gmail.com

DOI: 10.52711/0975-4377.2022.00047   

Address: M. Venkata Ramana*, V. Sowmya, K. Sangeetha, K. Nagapreethi, M. Mounika, S. Divya, A. Kanakalaxmi
Department of Analysis and QA, Surabhi Dayakar Rao College of Pharmacy, Rimmanaguda, Gajwel, Telangana, India.
*Corresponding Author

Published In:   Volume - 14,      Issue - 4,     Year - 2022


ABSTRACT:
Literature survey reveals that few spectrophotometric, high performance liquid chromatographic (HPLC)and High performance thin layer chromatographic (HPTLC)methods were reported for the estimation of Amlodipine and perindopril in bulk and combined pharmaceutical dosage forms. But there is no any stability indicating high performance liquid chromatography study is reported for amlodipine. So the present study was aimed to development of simple, accurate, rapid and economical HPLC stability indicating assay method were established for the determination of Amlodipine and Peridopril in bulk and tablet dosage form. A High-performance liquid chromatograph WATERS, software: Empower 2,2695 separation module, 996 PDA detector, using Phenomenex Luna C18 (4.6mm×250mm) 5µm or equivalent column, with mobile phase composition of Methanol: Phosphate Buffer pH3.0 (70:30v/v) was used. The flow rate of 1.0ml min-1 and effluent was detected at 230nm. The retention time of Amlodipine and Perindopril was found to be 1.870min and 2.499minutes respectively. Linearity was observed over concentration range of 10-50µg ml-1 for Amlodipine and 16-80µg ml-1 for Perindopril respectively. The accuracy of the proposed method was determined by recovery studies and the Amlodipine was found to be 99.1% and Perindopril was found to be 98.8% respectively. The proposed method is applicable to routine analysis of Amlodipine and Perindopril in bulk and pharmaceutical formulations. The proposed method was validated for various ICH parameters like linearity, limit of detection, limits of quantification, accuracy, precision, range and specificity.


Cite this article:
M. Venkata Ramana, V. Sowmya, K. Sangeetha, K. Nagapreethi, M. Mounika, S. Divya, A. Kanakalaxmi. Method Development and Validation for the estimation of Amlodipine and Perindopril in Bulk and Pharmaceutical Dosage Form. Research Journal of Pharmaceutical Dosage Forms and Technology. 2022; 14(4):289-2. doi: 10.52711/0975-4377.2022.00047

Cite(Electronic):
M. Venkata Ramana, V. Sowmya, K. Sangeetha, K. Nagapreethi, M. Mounika, S. Divya, A. Kanakalaxmi. Method Development and Validation for the estimation of Amlodipine and Perindopril in Bulk and Pharmaceutical Dosage Form. Research Journal of Pharmaceutical Dosage Forms and Technology. 2022; 14(4):289-2. doi: 10.52711/0975-4377.2022.00047   Available on: https://rjpdft.com/AbstractView.aspx?PID=2022-14-4-6


REFERENCES:
1.    Akhilesh Gupta, Swati Rawat, Mayuri Gandhi, Jaydeep Singh Yadav. Method Development and Acid Degradation Study of Doxofylline by RP-HPLC and LC-MS/MS. Asian J. Pharm. Ana. 1(1): Jan.-Mar. 2011; Page 10-13.
2.    Md. Ahsanul Haque, Mohammad Shahriar, Most. Nazma Parvin, S. M. Ashraful Islam. Validated RP-HPLC Method for Estimation of Ranitidine Hydrochloride, Domperidone and Naproxen in Solid Dosage Form. Asian J. Pharm. Ana. 1(3): July-Sept. 2011; Page 59-63.
3.    Sharmin Reza Chowdhury, MahfuzaMaleque, Mahbubul Hoque Shihan. Development and Validation of a Simple RP-HPLC Method for Determination of Caffeine in Pharmaceutical Dosage Forms. Asian J. Pharm. Ana. 2(1): Jan.-Mar. 2012; Page 01-04
4.    Revathi R., Venkata Naga Suresh P., Koteswara Rao M., Ethiraj T., Rajarajan S. Development and Validation of RP-HPLC Method for Content Analysis of Didanosine in Dosage Form. Asian J. Pharm. Ana. 2(4): Oct. - Dec. 2012; Page 118-121.
5.    D. Sridharan, Umarani A. Thenmozhi, L. Pavan Kumar, AswaniDuttChintalapati, M. Venkata Ramanaiah, YelikaPhanikishore. Development and Validation of UV Spectrophotometric Method of Darifenacin Hydrobromide in Bulk and Tablet Dosage Form. Asian J. Pharm. Ana. 1(3): July-Sept. 2011; Page 43-45.
6.    L. Satyanarayana, S.V. Naidu, M. Narasimha Rao, Reddy Suma Latha. The Estimation of Nilotinib in Capsule dosage form by RP-HPLC. Asian J. Pharm. Ana. 1(4): Oct. - Dec. 2011; Page 100-102.
7.    A. Thenmozhi, D. Sridharan, S. Veeramani, M. Palanivelu. An RP-HPLC Method for the estimation of Dexibuprofen in Pharmaceutical Tablet Dosage Form. Asian J. Pharm. Ana. 1(4): Oct. - Dec. 2011; Page 98-99.
8.    Mahmoud M. Sebaiy, Abdullah A. El-Shanawany, Sobhy M. El-Adl, Lobna M. Abdel-Aziz, Hisham A. Hashem. Rapid RP-HPLC Method for Simultaneous Estimation of Norfloxacin and Tinidazole in Tablet Dosage Form. Asian J. Pharm. Ana. 1(4): Oct. - Dec. 2011; Page 79-84.
9.    Md. Ahsanul Haque, Mohammad Shahriar, Most. Nazma Parvin, S. M. Ashraful Islam. Validated RP-HPLC Method for Estimation of Ranitidine Hydrochloride, Domperidone and Naproxen in Solid Dosage Form. Asian J. Pharm. Ana. 1(3): July-Sept. 2011; Page 59-63.
10.    Akhilesh Gupta, Jaydeep Singh Yadav, Swati Rawat, Mayuri Gandhi. Method Development and Hydrolytic Degradation Study of Doxofylline by RP-HPLC and LC-MS/MS. Asian J. Pharm. Ana. 1(1): Jan.-Mar. 2011; Page 14-18.

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DOI: 10.5958/0975-4377 


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