ABSTRACT:
The present study was aimed to evaluate the anticancer activity of various leaf extracts of Psidiumguajava against the breast cancer cell line (MCF-7). Trypan Blue dye exclusion test is used to determine the number of viable cells present in a cell suspension. The theory is based on the assumption that live cells possess intact cell membranes that exclude certain dyes, such as trypan blue, Eosin, or propidium, whereas dead cells do not.If the cell suspension is simply mixed with the dye and then visually checked to see if the cells are taking or lacking dye. An effective cell would have a clear cytoplasm and a blue cytoplasm would have a nonviable cell. Periodic tests of cell viability provide an early measure of fresh cell consistency prior freezing. Excellent viability beyond or equal to 95%. Results of the study showed a high potential for anticancer activity in the human cancer cell line MCF-7 in different leaf extracts of Psidiumguajava. Further detailed investigation of the plant active components for an accurate mechanism of action will make a significant contribution to the development of new pharmaceuticals.
Cite this article:
Akshay R. Yadav, Shrinivas K. Mohite. Anticancer Activity of Psidium guajava Leaf Extracts on Breast Cancer Cell Line. Res. J. Pharma. Dosage Forms and Tech.2020; 12(4):298-300. doi: 10.5958/0975-4377.2020.00049.X
REFERENCES:
1. Mohammad S. Anticancer agents from medicinal plants. Bangladesh J Pharmacol.2006; 1: 35-41.
2. Yadav A, Mohite S. Screening of In-vitro anti-inflammatory and Antibacterial assay of MalvastrumCoromandelianum. International Journal of Pharma Sciences and Research. 2020; 11(4): 68-70.
3. Kinghorn AD, Farnsworth NR, Soejarto DD. Novel strategies for the discovery of plant derived anticancer agents. Pharmaceutic Biol. 2003; 23: 53-67.
4. Parinitha M, Srinivasa BH, Shivanna MB. Medicinal plant wealth of local communities in some villages in Shimoga distinct of Karnataka.India J Ethnopharmacol.2005; 98: 307-312.
5. Vijayabaskaran M, Venkateswaramurthy N, Arif pasha MD, Babu G, Sivakumar P, Perumal P, Jayakar B. In vitro cytotoxic effect of ethanolic extract of Pseudarthriaviscidalinn.Int J Pharmacy Pharm Sci. 201; 2(3): 93-94.
6. Vishnu Priya P, Radhika K, SrinivasaRao A. In vitro anticancer activity of aqueous and acetone extracts of Tridaxprocumbens leaf on PC 3 cell lines. Int J Pharm Pharm Sci., 3(4), 2011: 1-4.
7. Sanjay Patel, NiravGheewala, Ashok Suthar, Anand Shah. In vitro cytotoxicity activity of Solanumnigrum extract against HELA cell line and VERO cell line. Int J Pharmacy Pharm Sci. 2009; 1(1): 38-46.
8. Abdullaev FI. Plant derived agents against cancer, In: Gupta, S. K., editor, Pharmacology and therapeutics in the new millennium, Narosa Publishing House, New Delhi, India, 2001: 345-354.
9. Cragg GM, Newman DJ. Plants as source of anticancer agents. J Ethnopharmacol.2005; 10: 72-79.
10. Cell viability testing with tryphan exclusion method, social & scientific systems, INC. National institute of environmental health sciences:1-2.
11. Yadav A, Mohite S, Magdum C. Synthesis, Characterization and Biological Evaluation of Some Novel 1,3,4-Oxadiazole Derivatives as Potential Anticancer Agents. Int J Sci Res Sci Technol. 2020; 7 (2): 275-282.
12. Freitas B, Almeida V, Pinto M, Mourao F, Massensini A, Filho O, Viera E, Melo G. Tryphan blue exclusion assay by flow cytometry. Braz J Med Biol. 2014; 47(4): 307-315.
13. Yadav A, Mohite S. Anticancer Activity and In-Silico ADMET Analysis of MalvastrumCoromandelianum. International Journal of Pharma Sciences and Research. 2020; 11(5): 71-73.
14. Subba RVV, Sirsi M. Effect of Abrusprecatorius L, on experimental tumors, Cancer Res. 1969; 29:1447-1451.