Author(s): Subhadeep Ganguly, Kunja Bihari Satapathy, Ajit Kumar Banik

Email(s): res_biol@rediffmail.com

DOI: Not Available

Address: Subhadeep Ganguly1, Kunja Bihari Satapathy1* and Ajit Kumar Banik2
1Post-Graduate Department of Botany, Utkal University, Vani Vihar, Bhubaneswar-751004, Odisha
2Department of Chemical Engineering, Biochemical Engineering Division, Biotechnology Laboratory, University of Calcutta, West Bengal India
*Corresponding Author

Published In:   Volume - 6,      Issue - 1,     Year - 2014


ABSTRACT:
Corynebacterium glutamicum X1 , a L-glutamic acid producing wild strain which accumulated mild amount of L-methionine (only 0.6 mg/ml) in the broth ,was subjected to mutational treatments using EMS and UV irradiations to develop a high L-methionine producing mutant. High L-methionine yielding strain Corynebacterium glutamicum X168 was selected in this study which was then subjected to protoplast fusion with a multiple L-methionine analogue-resistant strain Corynebacterium glutamicum X124. A high L-methionine yielding, multiple L-methionine analogue resistant mutant Corynebacterium glutamicum X300 was derived in this study which accumulated 9.6 mg /ml L-methionine in the fermentation broth.


Cite this article:
Induced Mutation, Development of Multiple Analogue Resistant Strain and Protoplast Fusion for L-Methionine Fermentation by Corynebacterium glutamicum. Subhadeep Ganguly, Kunja Bihari Satapathy, Ajit Kumar Banik. Res. J. Pharm. Dosage Form. & Tech. 6(1): Jan.-Mar. 2014; Page 01-06.


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RNI: Not Available                     
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