1. INTRODUCTION:

One of the most famous Ayurvedic health supplement called Chyawanprash is made up of a super concentrated blend of nutrient-rich herbs along with various minerals¹. In Ayurveda, Chyawanprash is classified under the category of Rasayana^1,2. Chyawanprash is also spelled as chyavanaprash, chyavanaprasha, chyavanaprasam, and chyawanaprash. The word Chyawanprash is combination of two lexes, i.e. “Chyawan” and “Prasha”. Chyawan is the name of a sage, and it is also symbolizes ‘degenerative change’, while Prasha means a drug or foodstuff which is suitable for consumption¹. Chyawanprash is used traditionally to increase the vitality, vigor as well as for delaying the aging process and for maintaining the physique³.

The recipe of chyawanprash has remained India’s one of the most popular and widely used remedy for around past 2000 years, from the time of the Vedas^1,3,4. Chyawanprash is formulated by using and processing around 50 medicinal herbs, minerals sugar, honey, ghee, Indian gooseberry jam, etc^4,5,6. Chyawanprash contains many Ayurvedic ingredients which helps in boosting the immunity, protects against infections, as well as it provides nutrition and energy⁶. Chyawanprash holds multiple health benefits¹. It has been extensively used from ancient times as an Ayurvedic health supplement and as a medicine which increase immunity and longevity¹. Consumption of Chyawanprash has been a part of every Indian’s life from the day it was introduced, irrespective of sociocultural, political, and scientific factors¹. It is famous for its antiaging effects long before vitamins, minerals, and antioxidant supplements¹. Chyawanprash which is consists of Amla/Amalaki pulp as a base is considered to be the most effective Rasayana for sustaining homeostasis^1-7. Regular intake Chyawanprash helps maintains physiological functions as well as rejuvenates the whole body system^1,8. The foremost historically recorded recipe for Chyawanprash is reported in the Charaka Samhita, the ancient Ayurvedic classic, where it is appreciated as being superior to all other herbal rejuvenative tonics^1,9. Immune system is major and vital system of our body which protects our body from outside invaders like Bacteria, viruses, fungi, toxins, etc. every part of our body including our immune system functions better. When protected from environmental assaults and boostered by healthy living strategies such as exercise, eating healthy food etc.

2. MATERIALS AND METHODS:

2.1 MATERIALS:

2.1.1 Collection of samples:

Pueraria tuberosa (Bidarikand), Santalum album (White sandalwood), Justicia adhatoda (Vasaka), Anacyclus pyrethrum (Akarkara), Asparagus racemosus (Shatavari), Bacopa monnieri (Brahmi), Aegle marmelos (Bel), Kamal Kesar (Sacred Lotus), Nardostachys jatamansi (Jatamansi), Tribulus terrestris (Gokhru), Curcuma zedoaria (Kachur), Cyperus rotundus (Nagarmotha), Syzygium aromaticum (Cloves), Laurus nobilis (Bay leaf), Inula racemose (Pushkaramul), Pistacia integerrima (Kakadshingi), Aegle marmelo (Dashmool), Boerhavia diffusa (Punarnava), Withania somnifera (Ashwagandha), Tinospora cordifolia (Giloy), Ocimum tenuiflorum (Tulsi), Murraya koenigii (Curry), Zingiber officinale (Ginger), Glycyrrhiza glabra (Mulethi), Elettaria cardamomum (Elaichi), Mesua ferrea (Nagkesar), Madhu (Honey), Crocus sativus (Saffron), Piper (Small piper), Cinnamon cassia (Cinnamon bark), Phyllanthus emblica (Amla), Kishmish, Ghee, Banslochan, Sugar were collected from areas of Sangli district.

2.2 METHODS:

2.2.1 Preparations of Chyawanprash:

1kg of raw Phyllanthus emblica (Amla) was taken in a vessel and boiled on minimum flame for 1Hr. with sufficient amount water (Fig. 1A). After boiling remove the seeds from Phyllanthus emblica (Amla). Crush the pulp to make consistence paste (Fig. 1B). At next step, make powder of Pueraria tuberosa (Bidarikand), Santalum album (White sandalwood), Justicia adhatoda (Vasaka), Anacyclus pyrethrum (Akarkara), Asparagus racemosus (Shatavari), Bacopa monnieri (Brahmi), Aegle marmelos (Bel), Kamal Kesar (Sacred Lotus), Nardostachys jatamansi (Jatamansi), Tribulus terrestris (Gokhru), Curcuma zedoaria (Kachur), Cyperus rotundus (Nagarmotha), Inula racemose (Pushkaramul), Pistacia integerrima (Kakadshingi), Aegle marmelo (Dashmool), Boerhavia diffusa (Punarnava), Withania somnifera (Ashwagandha), Tinospora cordifolia (Giloy), Glycyrrhiza glabra (Mulethi), Mesua ferrea (Nagkesar), Chocolate Compound and mix all this equally. After mixing all the herbs, add Ghee and stir the mixture to form uniform paste (Fig. 1C). Then cook this mixture for half-hour and then add raisins (Fig. 1D). After the colour change, add the 1 cup honey and half kg sugar and again cook it half-hour (Fig. 1E). Add powdered flavoring agents, Syzygium aromaticum (Cloves), Laurus nobilis (Bay leaf), Zingiber officinale (Ginger), Elettaria cardamomum (Elaichi), Crocus sativus (Saffron), Piper (Small piper), Cinnamon cassia (Cinnamon bark). After adding all ingredients mix it uniformly and cool it (Fig. 1F). When Chyawanprash is ready then retain it for one day. After one day make small bolls of chyawanprash (Fig. 1G) and rap it in the aluminum paper (Fig. 1H).

Table 1. Formula for Chyawanprash.

Sr. No.	Ingredients	Quantity
1	Pueraria tuberosa	5gm
2	Santalum album	5gm
3	Justicia adhatoda	5gm
4	Anacyclus pyrethrum	5gm
5	Asparagus racemosus	5gm
6	Bacopa monnieri	5gm
7	Aegle marmelos	5gm
8	Kamal Kesar	5gm
9	Nardostachys jatamansi	5gm
10	Tribulus terrestris	5gm
11	Curcuma zedoaria	5gm
12	Cyperus rotundus	5gm
13	Syzygium aromaticum	5gm
14	Laurus nobilis	5gm
15	Inula racemose	5gm
16	Pistacia integerrima	5gm
17	Aegle marmelo	5gm
18	Boerhavia diffusa	5gm
19	Withania somnifera	5gm
20	Tinospora cordifolia	5gm
21	Ocimum tenuiflorum	5gm
22	Murraya koenigii	5gm
23	Zingiber officinale	5gm
24	Glycyrrhiza glabra	5 gm
25	Elettaria cardamomum	10 gm
26	Crocus sativus	5 gm
27	Piper	5 gm
28	Cinnamon cassia	3 gm
29	Phyllanthus emblica	1 kg
30	Madhu	30 gm
31	Mesua ferrea	5 gm
32	Kishmish	20 gm
33	Ghee	50 gm
34	Banslochan	5 gm
35	Sugar	0.5 kg
36	Chocolate Compound	q.s.

2.2.2 Evaluation of the Prepared Chyawanprash:

2.2.2.1 Ellagic test for phenol:

2gm chyawanprash was taken and treated with 4 drops of glacial acetic acid followed by 4 drops of NaNO ² Solution and shake the mixture.

2.2.2.2 Test for saponins:

2gm of the chyawanprash was diluted with 10ml distilled water and vigorously shaken well for 20 min in a graduated cylinder.

2.2.2.3 Ferric chloride Test for flavonoids:

5 drops of ferric chloride solution were added 2 gm chyawanprash.

2.2.2.4 Sulphuric acid test for glycosides:

2.5 gm chyawanprash, 2ml glacial acetic acid was added. This was followed by addition of one drop of FeCl₃ the was then acidified by drop wise addition of concreted H₂SO₄

2.2.2.5 Test for steroid:

2ml acetic anhydride was added to 1gm of chyawanprash. The solution was acidified by dropwise addition of sulphuric acid.

2.2.2.6 Wagner’s test for alkaloids:

5 drops of Wagner’s reagent were added to 2 gm chyawanprash. The mixture was warmed for one minute.

2.2.2.7 Braemer’s test for tannin:

2ml of % ferric chloride was added to 2 gm chyawanprash.

3. RESULTS:

Test	Observation	Result
Ellagic test for phenol 2gm chyawanprash+ 4 drops of glacial acetic acid + 4drops NaNO² shake the mixture	Muddy brown PPT appearance	Phenol is present
Test for saponins: 2gm of the chyawanprash diluted in 10 ml water shake it for 20min	1 cm layer of foam appears	Saponins are present
Ferric chloride Test for flavonoids 5 drops of FeCl₃ + 2 gm chyawanprash	Green colour turns blue	phenolic hydroxyl group
Sulphuric acid test for glycosides 2.5 gm chyawanprash + 2ml glacial acetic acid +FeCl₃ +H₂SO₄	Brown ring appears at liquid interface	Presence of glycosides
Test for steroid Test 2 gm of sample + 2ml acetic anhydride +H₂SO₄	Dark green or blue colour appears	Steroid is present
Wagner’s test for alkaloids Wagner’s reagent + 2gm sample warm it for 1 min	Reddish brown PPT appears	Alkaloids are present
Braemer’s test for tannin 2 gm sample + 2ml FeCl₃	Dark blue / greenish grey colour appears	Tannins are present

4. CONCLUSION AND DISCUSSION:

Chyawanprash is a most famous traditional Ayurvedic immune booster recipe. It has a long history of ethical origin, development, household preparation and usage. Chyawanprash has grown to industrial production and marketing in packed form to a large number of consume/patients like any food or health care product. My project work is based on chyawanprash include some modification in the form of candy, so that every consumer experience its therapeutic value in modern way the preparation also includes some excipient alternative then the original once which enhance the immune boosting activity. From the test and result we/I can conclude that the prepared formulation is very effective and ready to manufacture/made on industrial seal. The prepared chyawanprash is organic material and its quality is excellent. It does not contain any chemical substance. The new idea of making chyawanprash in chocolate form is just to increase the attractiveness of the product for the children it is good in test as well as in its nutrition value also the chyawanprash increase the immunity and also boost are health daily intake of it shows the real result, resist the body from diseases.

5. REFERENCES:

1. Sharma R, Martins N, Kuca K, Chaudhary A, Kabra A, Rao MM, Prajapati PK. Chyawanprash: a traditional Indian bioactive health supplement. Biomolecules. 2019; 9(5):161.

2. Rathi B, Wanjari A, Rajput D, Khan M, Rathi R. Pharmaceutical standardization of Chyawanprash prepared by two different methods. Journal of Indian System of Medicine. 2018; 6(4):179.

3. Bansal N, Parle M. Beneficial effect of chyawanprash on cognitive function in aged mice. Pharmaceutical Biology. 2011; 49(1):2-8.

4. Kumar A, Rinwa P, Kaur P. Chyawanprash: A wonder Indian rasayana from ayurveda to modern age. Crit. Rev. Pharm. Sci. 2012; 1:1-8.

5. Parle, M.; Bansal, N. Traditional medicinal formulation, Chyawanprash—A review. Ind. J. Tradit. Knowl. 2006, 5, 484–488.

6. Tamboli FA, More HN, Khairmode SS, Patil DR, Tambare PD, Shinde AJ, Jadhav NR, Bhandari SV. Chyawanprash: A traditional indian bioactive herbal medicinal formulation to boost immunity and restore youthfulness. Tropical Journal of Pharmaceutical and Life Sciences (TJPLS Journal). 2021; 8(1):21-8.

7. Panda, H. Handbook on Ayurvedic Medicines with Formulae, Processes and Their Uses; National Institute of Industrial Research: New Delhi, India, 2002 p. 10.

8. Rao, R.S.K. Encyclopaedia of Indian Medicine-Historical Perspective, Popular Prakashan: Bombay, India, 1985; Volume I, 25p.

9. Bates, D. Knowledge and the Scholarly Medical Traditions; Cambridge University Press: Cambridge, MA, USA, 1995; 325p.

10. Anonymous. The Ayurvedic Formulary of India; Ministry of Health & Family Welfare: New Delhi, India, 2003; p. 37.

11. Rathod S, Mali S, Shinde N, Aloorkar N. Cosmeceuticals and Beauty Care Products: Current trends with future prospects. Research Journal of Topical and Cosmetic Sciences. 2020; 11(1):45-51

12. Poonam Amrutia, Darshana Dave. Prevailing Tenor for Ayurveda in Views of Ayurvedic Practitioners. Asian J. Management; 2017; 8(4):1029-1036.

13. A. S. Suresh, Bhavna Pawar. A Study of Factors of Lifestyle and its impact on Nutraceutical Consumption: India Perspective. Asian Journal of Management. 2018; 9(1):203-211.

14. Suraj J. Patil, Shivani D. Patil, Pratibha B. Patil, Pranali S. Patil, Ganesh B. Vambhurkar, Indryani D. Raut. Evaluation of Standardization Parameters of Ayurvedic Marketed Polyherbal Formulation. Asian J. Pharm. Ana. 2018; 8(4): 220-226.

15. Alex Nemtsov, Pavel Kirshkov. The Study of Precision of ISO 3632-2 Method for Analysis of Herbal Medicines. Asian J. Pharm. Ana. 2020; 10(3):125-128.

16. P. Jaya Preethi. Herbal Medicine for Diabetes Mellitus: A Review. Asian J. Pharm. Res. 3(2): April- June 2013; Page 57-70.

17. Hindustan Abdul Ahad, et al. Immuno-Boosters as Health Accelerants to Tackle Viral Infections. Asian Journal of Pharmaceutical Research. 2021; 11(3):212-6.

18. Bharti Ahirwar. Evaluation of stability study of Ayurvedic formulation Vasavaleha. Asian J. Res. Pharm. Sci. 3(1): Jan.-Mar. 2013; Page 01-04.

19. Seema Thakur, Neha Srivastava. Nutraceuticals: A Review. Asian J. Res. Pharm. Sci. 2016; 6(2): 85-94.

20. Sushmita V. Patil, Vaishnavi K. Patil, Paresh A. Patil. Review on Herbal medicines of Alzheimer’s disease. Asian J. Res. Pharm. Sci. 2020; 10(3):171-177.

Received on 25.10.2021 Modified on 29.01.2022

Res. J. Pharma. Dosage Forms and Tech.2022; 14(2):123-126.

DOI: 10.52711/0975-4377.2022.00019