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Research Journal of Pharmaceutical Dosage Forms and Technology
ISSN: 0975-4377(Online), 0975-234X(Print)
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New Spectrophotometric Method for Assay of Amphotericin B in Bulk and Its Pharmaceutical Formulations
Mohan Krishna Lokireddy
Jayachandra Reddy P
M. Srinivasa Murthy
Mohan Krishna Lokireddy1*, Jayachandra Reddy P2, M. Srinivasa Murthy1
1Vignan Institute of Pharmaceutical Sciences, Deshmukhi, Nalgonda, Andhrapradesh, India.
2Krishna Teja Pharmacy College, Tirupati, India.
Two simple and sensitive visible spectrophotometric methods (A and B) have been developed for the determination of Amphotericin B (AMP) in bulk and pharmaceutical formulations. These methods are based on the Method A was developed based on the formation of a colored condensation reaction with p-dimethyl amino cinnamaldehyde (PDAC) under acidic conditions (?max 490 nm), and method B based on the oxidative coupling reaction with DCQC (2,6-dichloroquinone N-chlorimide) (?max 530nm) . Beer’s law limits, precision and accuracy of these methods are checked by the UV reference method. The results obtained are reproducible and are statistically validated and so found to be suitable for the assay of in bulk and pharmaceutical formulations.
Mohan Krishna Lokireddy, Jayachandra Reddy P, M. Srinivasa Murthy. New Spectrophotometric Method for Assay of Amphotericin B in Bulk and Its Pharmaceutical Formulations. Res. J. Pharm. Dosage Form. and Tech. 6(1): Jan.-Mar. 2014; Page 15-17.
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