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Research Journal of Pharmaceutical Dosage Forms and Technology
ISSN: 0975-4377(Online), 0975-234X(Print)
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Induced Mutation, Development of Multiple Analogue Resistant Strain and Protoplast Fusion for L-Methionine Fermentation by Corynebacterium glutamicum
Kunja Bihari Satapathy
Ajit Kumar Banik
Subhadeep Ganguly1, Kunja Bihari Satapathy1* and Ajit Kumar Banik2
1Post-Graduate Department of Botany, Utkal University, Vani Vihar, Bhubaneswar-751004, Odisha
2Department of Chemical Engineering, Biochemical Engineering Division, Biotechnology Laboratory, University of Calcutta, West Bengal India
Corynebacterium glutamicum X1 , a L-glutamic acid producing wild strain which accumulated mild amount of L-methionine (only 0.6 mg/ml) in the broth ,was subjected to mutational treatments using EMS and UV irradiations to develop a high L-methionine producing mutant. High L-methionine yielding strain Corynebacterium glutamicum X168 was selected in this study which was then subjected to protoplast fusion with a multiple L-methionine analogue-resistant strain Corynebacterium glutamicum X124. A high L-methionine yielding, multiple L-methionine analogue resistant mutant Corynebacterium glutamicum X300 was derived in this study which accumulated 9.6 mg /ml L-methionine in the fermentation broth.
Corynebacterium glutamicum , L-glutamic acid , L-methionine, analogue resistant.
Induced Mutation, Development of Multiple Analogue Resistant Strain and Protoplast Fusion for L-Methionine Fermentation by Corynebacterium glutamicum. Subhadeep Ganguly, Kunja Bihari Satapathy, Ajit Kumar Banik. Res. J. Pharm. Dosage Form. & Tech. 6(1): Jan.-Mar. 2014; Page 01-06.
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