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Research Journal of Pharmaceutical Dosage Forms and Technology
ISSN: 0975-4377(Online), 0975-234X(Print)
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Simultaneous Determination of Paracetamol and Metoclopramide in Antimigraine Pharmaceutical Formulations
Abd El-Aziz B. Abd El-Aleem
Shaban M. Khalile
Amr M. Badawy
Omneya K. El-Naggar
Abd El-Aziz B. Abd El-Aleem1, Shaban M. Khalile2, Amr M. Badawy1 and Omneya K. El-Naggar2*
1Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt
2National Organization for Drug Control And Research (NODCAR), Giza, Egypt
This paper describes sensitive, accurate and precise TLC- densitometric and high performance liquid chromatographic (HPLC) methods for simultaneous determination of paracetamol and metoclopramide in pharmaceutical formulation. The TLC method employed aluminum TLC plates precoated with silica gel F254 as the stationary phase and ethylacetate/ methanol/ ammonia (85:10:5 v/v/v) as the mobile phase, where the chromatogram was scanned at 254 nm. The developed HPLC method used a Zorbax C18 column with isocratic elution. The mobile phase composed of phosphate buffer pH 4.0: methanol (75:25 v/v) at flow rate of 1.0 ml/min. Quantitation was achieved with UV detection at 273 nm. The methods were validated according to the International Conference on Harmonization (ICH) guidelines. The selectivity of the proposed methods was tested using laboratory-prepared mixtures. The developed methods were successfully applied for the determination paracetamol and metoclopramide in bulk powder and combined dosage form.
High-performance liquid chromatography, Thin layer Chromatography, antimigraine drugs, paracetamol, metoclopramide.
Abd El-Aziz B. Abd El-Aleem, Shaban M. Khalile, Amr M. Badawy, Omneya K. El-Naggar. Simultaneous Determination of Paracetamol and Metoclopramide in Antimigraine Pharmaceutical Formulations. Research J. Pharma. Dosage Forms and Tech. 2013; 5(5): 269-273.
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