Volume No. :   5

Issue No. :  1

Year :  2013

ISSN Print :  0975-234X

ISSN Online :  0975-4377


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Development and Validation of RP-HPLC Method for Estimation of Mycophenolate Mofetil in Bulk and Pharmaceutical Dosage Form



Address:   Rajendra Prasad. K.* and Kathirvel S.
Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Amaravathi road, Guntur. A.P.
*Corresponding Author:
DOI No:

ABSTRACT:
A simple, rapid, sensitive and precise high performance liquid chromatography (HPLC) method has been developed for the estimation of Mycophenolate mofetil in bulk and pharmaceutical dosage form. In this method RP-C18 column (250mmx4.6mm I.D and5µm particle size) with mobile phase consisting of acetonitrile and 0.02M phosphate buffer in the ratio of 50:50 v/v in isocratic mode was used. The detection wavelength is 230 nm and the flow rate is 1 ml/min. In the range of 10-60 µg/ml, the linearity of Mycophenolate mofetil shows a correlation coefficient of 0.999. The proposed method was validated by determining sensitivity, accuracy, precision and system suitability parameters. The mobile phase selected for the method is simple, fast, accurate and precise and hence can be applied for routine quality control analysis of Mycophenolate mofetil in bulk and its pharmaceutical dosage form. The method was fully validated according to ICH guidelines.
KEYWORDS:
Mycophenolate mofetil, High performance liquid chromatography, Validation
Cite:
Rajendra Prasad. K., Kathirvel S. Development and Validation of RP-HPLC Method for Estimation of Mycophenolate Mofetil in Bulk and Pharmaceutical Dosage Form. Research J. Pharma. Dosage Forms and Tech. 2013; 5(1): 42-45.
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