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Research Journal of Pharmaceutical Dosage Forms and Technology
ISSN: 0975-4377(Online), 0975-234X(Print)
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Development and Validation of visible Spectrophotometric method of Tapentadol Hydrochloride in bulk and Pharmaceutical dosage form
Madhu Babu K.
Madhu Babu K., Kathirvel S.*
Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Amaravathi Road, Guntur, Andhra Pradesh – 522002
A simple, sensitive, accurate spectrophotometric method has been developed for the estimation of Tapentadol hydrochloride in bulk and pharmaceutical formulation. The method is based on the formation of blue colored chromogen with Folin-Ciocalteu reagent in the presence of NaOH and obeys beer’s law in the range of 5 - 35µg/mL exhibiting the maximum absorbance at 763nm. The limit of detection and quantification were found to be 0.76µg/mL and 2.33µg/mL respectively. The calibration curve demonstrated a linear relationship between the absorbance and concentration, with the correlation coefficient higher than 0.9999. The Regression Equation of the curve was Y=0.0248x-0.0436. The method was fully validated according to ICH guidelines.
Tapentadol hydrochloride, Folin-Ciocalteu (FC) reagent, NaOH, Validation, ICH guidelines.
Madhu Babu K., Kathirvel S. Development and Validation of visible Spectrophotometric method of Tapentadol Hydrochloride in bulk and Pharmaceutical dosage form. Research J. Pharma. Dosage Forms and Tech. 2012; 4(6): 328-331.
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