Volume No. :   2

Issue No. :  1

Year :  2010

ISSN Print :  0975-234X

ISSN Online :  0975-4377


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Isolation and Characterization of Antibiotic Production from Soil Isolates by Fermentation



Address:   Isolation and Characterization of Antibiotic Production from Soil Isolates by Fermentation Chandrashekhara S2*, BK Nanjwade1, PS Goudanavar2, FV Manvi1 Shamrez Ali M1
1KLES’s College of Pharmaceutical Sciences, K.L.E. University, Nehru Nagar, Belgaum, Karnataka, India.
2KLES’s College of Pharmaceutical Sciences, Nipani, Karnataka, India,
*Corresponding Author
DOI No:

ABSTRACT:
In screening of new antibiotics, several actinomycetes were isolated from soil samples. Crowded plate technique was used for the isolation of actinomycetes. The morphological and cultural characterization of A-4 strain was performed. In medium formulation study for A-4 and A-4 mutant, various carbon and nitrogen sources were tested for maximum antibiotic production using zone of inhibition and packed cell volume (%) as parameters. Various fermentation conditions like pH, temperature and DO2 were also optimized for the maximal production of antibiotic from both A-4 and A-4 mutant. All medium formulation as well as bioprocess parameters for A-4 and A-4 mutant strains was compared. Some actinomycetes strains, showed promising antimicrobial scores against different strains of bacteria and fungi. From the six strains selected, one strain designated as A-4 showed maximum antimicrobial property against gram positive and gram negative strains as well as various fungi. Morphological and cultural studies showed that A-4 is belongs to actinomycete genus.. The strain A-4 and A-4 mutant was found to be having better antimicrobial activity in comparison with other soil isolates of actinomycetes.
KEYWORDS:
Actinomycete, Antibiotic, Crowded plate technique, Zone of Inhibition, Fermentation.
Cite:
Chandrashekhara S, BK Nanjwade, PS Goudanavar, FV Manvi, Shamrez Ali M. Isolation and Characterization of Antibiotic Production from Soil Isolates by Fermentation. Research J. Pharma. Dosage Forms and Tech. 2010; 2(1): 32-36 .
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